Hydroxyecdysone CAS 5289-74-7

About Hydroxyecdysone

Hydroxyecdysone (20-hydroxyecdysone, 20E, beta-ecdysone, CAS 5289-74-7) is a polyhydroxylated steroid hormone belonging to the ecdysteroid family. It was first identified as the molting hormone in insects, where it regulates metamorphosis and reproduction. In plants, hydroxyecdysone serves as a defense compound against insect herbivory.

Commercial hydroxyecdysone is typically extracted from plants such as Cyanotis vaga, Cyanotis arachnoidea, or Rhaponticum carthamoides. The compound has a characteristic steroid nucleus with seven hydroxyl groups, contributing to its high water solubility compared to other steroid hormones. In mammalian systems, hydroxyecdysone has been studied for its effects on protein synthesis, muscle growth, and metabolic regulation.

Specifications

Applications

Sports Nutrition & Bodybuilding

Dietary supplement ingredient marketed for muscle growth, strength gains, and athletic performance. Hydroxyecdysone is believed to stimulate protein synthesis through activation of the PI3K/Akt pathway and may enhance nitrogen retention. Typical serving size is 100-500 mg per day. Often formulated with whey protein, creatine, or other muscle-building ingredients in pre-workout and post-workout supplements.

Nutraceuticals & General Health

Ingredient in anti-aging, vitality, and metabolic support supplements. Hydroxyecdysone has been studied for its potential effects on blood sugar regulation, lipid metabolism, and immune function. Used in combination formulas with adaptogens such as rhodiola, ashwagandha, and ginseng.

Pharmaceutical Research

Research compound for studying steroid hormone signaling, ecdysteroid receptor interactions, and muscle wasting conditions. Investigated for potential therapeutic applications in sarcopenia, cachexia, and metabolic disorders. Used as a positive control in insect physiology and developmental biology research.

Agriculture & Aquaculture

Growth promoter and molt regulator in shrimp and crustacean aquaculture. Hydroxyecdysone supplementation can synchronize molting, improve survival rates, and enhance growth in farmed shrimp and prawns. Also used in insect rearing for research and biological control programs.

Cosmetics & Personal Care

Emerging ingredient in anti-aging and skin firming products. Hydroxyecdysone may support collagen synthesis and improve skin elasticity. Used in small concentrations (0.01-0.1%) in premium skincare formulations.

Quality Control & Documentation

Each batch is tested before release:

1. Hydroxyecdysone content by high-performance liquid chromatography (HPLC-UV)
2. Identity verification by UV spectroscopy and mass spectrometry
3. Specific rotation measurement by polarimetry
4. Moisture content by Karl Fischer titration
5. Heavy metal analysis by ICP-OES
6. Microbiological testing (total plate count, yeast and mold, E. coli, Salmonella)
7. Pesticide residue screening (for plant-derived material)

Available documentation includes COA, TDS, SDS/MSDS, HPLC chromatograms, and heavy metal test reports. Product is manufactured in facilities compliant with ISO 9001 and HACCP standards.

Storage & Handling

Store in a cool, dry, dark place at temperatures below 25°C. Keep containers tightly sealed under inert gas (nitrogen) to prevent oxidation and moisture absorption. Protect from light, heat, and strong oxidizing agents. Use amber glass or food-grade plastic containers. Shelf life is 24 months under proper storage conditions.

Wear protective gloves, safety glasses, and dust mask when handling. Avoid inhalation of dust and prolonged skin contact. Hydroxyecdysone is a research compound and should be handled according to standard laboratory practices. In case of eye contact, flush with water for 15 minutes and seek medical attention.

Frequently Asked Questions

Is hydroxyecdysone a steroid?

Hydroxyecdysone has a steroid chemical structure but is not an anabolic androgenic steroid. It does not bind to androgen receptors and does not cause the side effects associated with anabolic steroids. It is classified as a phytoecdysteroid and is legal as a dietary supplement ingredient in most countries.

What is the difference between ecdysone and hydroxyecdysone?

Ecdysone (C27H44O6) has six hydroxyl groups, while 20-hydroxyecdysone (C27H44O7) has an additional hydroxyl group at position 20. Hydroxyecdysone is the more biologically active form and is the primary commercial product. Most supplements labeled “ecdysone” actually contain 20-hydroxyecdysone.

Can hydroxyecdysone be detected in drug testing?

Hydroxyecdysone is not currently listed as a banned substance by WADA or most sports governing bodies. However, regulations may change, and athletes should verify current status with their relevant anti-doping agency. Standard drug tests do not typically screen for ecdysteroids.

What is the minimum order quantity?

Standard packaging includes 1 kg, 5 kg, and 25 kg drums. Smaller sample sizes (10-100 g) are available for research and formulation development. Full kilogram pricing is available for bulk orders. Lead time is typically 1-3 weeks for standard grades.